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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003845-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003845-M01, RRID:AB_425519
- Product name
- KRAS monoclonal antibody (M01), clone 3B10-2F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KRAS.
- Antigen sequence
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPT
IEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQ
YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKD
SEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIP
FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGK
KKKKKSKTKCVIM- Isotype
- IgG
- Antibody clone number
- 3B10-2F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Evidence for aldosterone-dependent growth of renal cell carcinoma.
Overexpression of DDX43 mediates MEK inhibitor resistance through RAS Upregulation in uveal melanoma cells.
KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy.
Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
King S, Bray S, Galbraith S, Christie L, Fleming S
International journal of experimental pathology 2014 Aug;95(4):244-50
International journal of experimental pathology 2014 Aug;95(4):244-50
Overexpression of DDX43 mediates MEK inhibitor resistance through RAS Upregulation in uveal melanoma cells.
Ambrosini G, Khanin R, Carvajal RD, Schwartz GK
Molecular cancer therapeutics 2014 Aug;13(8):2073-80
Molecular cancer therapeutics 2014 Aug;13(8):2073-80
KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy.
Valtorta E, Misale S, Sartore-Bianchi A, Nagtegaal ID, Paraf F, Lauricella C, Dimartino V, Hobor S, Jacobs B, Ercolani C, Lamba S, Scala E, Veronese S, Laurent-Puig P, Siena S, Tejpar S, Mottolese M, Punt CJ, Gambacorta M, Bardelli A, Di Nicolantonio F
International journal of cancer 2013 Sep 1;133(5):1259-65
International journal of cancer 2013 Sep 1;133(5):1259-65
Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
Fuentes-Calvo I, Blázquez-Medela AM, Santos E, López-Novoa JM, Martínez-Salgado C
PloS one 2010 Jan 14;5(1):e8703
PloS one 2010 Jan 14;5(1):e8703
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
