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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- MA5-34802 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 2 Recombinant Rabbit Monoclonal Antibody (JG39-64)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, MCF-7, SK-OV-3, LOVO, rat testis tissue, human liver cancer tissue, mouse testis tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG39-64
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nectin 2 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nectin 2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 2 in paraffin-embedded rat testis tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 2 in paraffin-embedded mouse testis tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 2 in paraffin-embedded human liver cancer tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Nectin 2 in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Nectin 2 in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
