19937-1-AP

antibody from Proteintech Group

Targeting: CYP2E1 CYP2E

Provider product page for 19937-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [59]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 19937-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#19937-1-AP, RRID:AB_10646444
Product name: CYP2E1-Specific antibody
Antibody type: Polyclonal
Description: CYP2E1-Specific antibody (Cat. #19937-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CYP2E1 protein structure - 19937-1-AP shown in red.

Submitted references Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism.

Xue M, Liang H, Zhou Z, Liu Y, He X, Zhang Z, Sun T, Yang J, Qin Y, Qin K
Food & nutrition research 2021;65

TA allele of rs2070673 in the CYP2E1 gene is associated with lobular inflammation and nonalcoholic steatohepatitis in patients with biopsy-proven nonalcoholic fatty liver disease.

Ma HL, Chen SD, Zheng KI, Yu Y, Wang XX, Tang LJ, Li G, Rios RS, Huang OY, Zheng XY, Xu RA, Targher G, Byrne CD, Wang XD, Chen YP, Zheng MH
Journal of gastroenterology and hepatology 2021 Oct;36(10):2925-2934

Compromised glutathione synthesis results in high susceptibility to acetaminophen hepatotoxicity in acatalasemic mice.

Ogino N, Nagaoka K, Tomizuka K, Matsuura-Harada Y, Eitoku M, Suganuma N, Ogino K
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Oct;156:112509

Hepatoprotective effects of ginsenoside Rk3 in acetaminophen-induced liver injury in mice by activation of autophagy.

Qu L, Fu R, Ma X, Fan D
Food & function 2021 Oct 4;12(19):9128-9140

MicroRNA-29b ameliorates hepatic inflammation via suppression of STAT3 in alcohol-associated liver disease.

Zhou K, Yin F, Li Y, Ma C, Liu P, Xin Z, Ren R, Wei S, Khan M, Wang H, Zhang H
Alcohol (Fayetteville, N.Y.) 2021 Oct 22;99:9-22

Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats.

Tan B, Chen J, Qin S, Liao C, Zhang Y, Wang D, Li S, Zhang Z, Zhang P, Xu F
Chemical research in toxicology 2021 Jul 19;34(7):1759-1768

Allicin alleviated acrylamide-induced NLRP3 inflammasome activation via oxidative stress and endoplasmic reticulum stress in Kupffer cells and SD rats liver.

Nan B, Yang C, Li L, Ye H, Yan H, Wang M, Yuan Y
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Feb;148:111937

Triptolide regulates oxidative stress and inflammation leading to hepatotoxicity via inducing CYP2E1.

Jiang HY, Bao YN, Lin FM, Jin Y
Human & experimental toxicology 2021 Dec;40(12_suppl):S775-S787

Protecting mitochondria via inhibiting VDAC1 oligomerization alleviates ferroptosis in acetaminophen-induced acute liver injury.

Niu B, Lei X, Xu Q, Ju Y, Xu D, Mao L, Li J, Zheng Y, Sun N, Zhang X, Mao Y, Li X
Cell biology and toxicology 2021 Aug 17;

Gut microbiota accelerates cisplatin-induced acute liver injury associated with robust inflammation and oxidative stress in mice.

Gong S, Feng Y, Zeng Y, Zhang H, Pan M, He F, Wu R, Chen J, Lu J, Zhang S, Yuan S, Chen X
Journal of translational medicine 2021 Apr 13;19(1):147

Pyruvate Kinase M2 Tetramerization Protects against Hepatic Stellate Cell Activation and Liver Fibrosis.

Zheng D, Jiang Y, Qu C, Yuan H, Hu K, He L, Chen P, Li J, Tu M, Lin L, Chen H, Lin Z, Lin W, Fan J, Cheng G, Hong J
The American journal of pathology 2020 Nov;190(11):2267-2281

circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury.

Wang Z, Zhao Y, Sun R, Sun Y, Liu D, Lin M, Chen Z, Zhou J, Lv L, Tian X, Yao J
Cell death & disease 2020 Nov 6;11(11):953

Intrabody against prolyl hydroxylase 2 ameliorates acetaminophen-induced acute liver injury in mice via concomitant promotion of angiogenesis and redox homeostasis.

Zhao L, Zhao J, Gao R, Tian Y, Zhang Y, Tang W, Jiang Y, Li C, Wang M, Yang F, Li G
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Mar;123:109783

Intestinal Epithelial Chemokine (C-C Motif) Ligand 7 Overexpression Enhances Acetaminophen-Induced Hepatotoxicity in Mice.

Niu M, Luo Z, Gong S, Win S, Kaplowitz N, Jiang Y, Chen P
The American journal of pathology 2020 Jan;190(1):57-67

Ferroptosis driven by radical oxidation of n-6 polyunsaturated fatty acids mediates acetaminophen-induced acute liver failure.

Yamada N, Karasawa T, Kimura H, Watanabe S, Komada T, Kamata R, Sampilvanjil A, Ito J, Nakagawa K, Kuwata H, Hara S, Mizuta K, Sakuma Y, Sata N, Takahashi M
Cell death & disease 2020 Feb 24;11(2):144

Effects of taraxasterol against ethanol and high-fat diet-induced liver injury by regulating TLR4/MyD88/NF-κB and Nrf2/HO-1 signaling pathways.

Li Z, Lian Y, Wei R, Jin L, Cao H, Zhao T, Ma X, Zhong M, Gao Y, Zhang K
Life sciences 2020 Dec 1;262:118546

Knockdown of Long Noncoding RNAs Hepatocyte Nuclear Factor 1α Antisense RNA 1 and Hepatocyte Nuclear Factor 4α Antisense RNA 1 Alters Susceptibility of Acetaminophen-Induced Cytotoxicity in HepaRG Cells.

Chen L, Wang P, Manautou JE, Zhong XB
Molecular pharmacology 2020 Apr;97(4):278-286

Chronic intake of high-dose of blueberry leaf extract does not augment the harmful effects of ethanol in rats.

Yamasaki K, Sugamoto K, Arakawa T, Nishiyama K, Yamasaki M
PeerJ 2019;7:e6989

Inhibitory Effect of Corilagin on miR-21-Regulated Hepatic Fibrosis Signaling Pathway.

Zhou X, Xiong J, Lu S, Luo L, Chen ZL, Yang F, Jin F, Wang Y, Ma Q, Luo YY, Wang YJ, Zhou JB, Liu P, Zhao L
The American journal of Chinese medicine 2019;47(7):1541-1569

CCl₄-Induced Liver Injury Was Ameliorated by Qi-Ge Decoction through the Antioxidant Pathway.

Peng C, Zhou ZM, Li J, Luo Y, Zhou YS, Ke XH, Huang KE
Evidence-based complementary and alternative medicine : eCAM 2019;2019:5941263

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Yuan J, Chen C, Cui J, Lu J, Yan C, Wei X, Zhao X, Li N, Li S, Xue G, Cheng W, Li B, Li H, Lin W, Tian C, Zhao J, Han J, An D, Zhang Q, Wei H, Zheng M, Ma X, Li W, Chen X, Zhang Z, Zeng H, Ying S, Wu J, Yang R, Liu D
Cell metabolism 2019 Oct 1;30(4):675-688.e7

Neuroprotective Effect of Dioscin on the Aging Brain.

Qi Y, Li R, Xu L, Yin L, Xu Y, Han X, Peng J
Molecules (Basel, Switzerland) 2019 Mar 30;24(7)

Inhibition of the mitochondrial complex-1 protects against carbon tetrachloride-induced acute liver injury.

Hua H, Zhang Z, Qian Y, Yuan H, Ge W, Huang S, Zhang A, Zhang Y, Jia Z, Ding G
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jul;115:108948

Benzene-induced mouse hematotoxicity is regulated by a protein phosphatase 2A complex that stimulates transcription of cytochrome P4502E1.

Chen L, Guo P, Zhang H, Li W, Gao C, Huang Z, Fan J, Zhang Y, Li X, Liu X, Wang F, Wang S, Li Q, He Z, Li H, Chen S, Wu X, Ye L, Li Q, Tang H, Wang Q, Dong G, Xiao Y, Chen W, Li D
The Journal of biological chemistry 2019 Feb 15;294(7):2486-2499

Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Dong D, Qi Y, Xu L, Yin L, Xu Y, Han X, Zhao Y, Peng J
Food & function 2019 Aug 1;10(8):5240-5241

Protective effects of ginsenoside Rk3 against chronic alcohol-induced liver injury in mice through inhibition of inflammation, oxidative stress, and apoptosis.

Qu L, Zhu Y, Liu Y, Yang H, Zhu C, Ma P, Deng J, Fan D
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2019 Apr;126:277-284

Hepatoprotective Potential of Partially Hydrolyzed Guar Gum against Acute Alcohol-Induced Liver Injury in Vitro and Vivo.

Wu C, Liu J, Tang Y, Li Y, Yan Q, Jiang Z
Nutrients 2019 Apr 27;11(5)

Study on the potential way of hepatic cytotoxicity of N,N-dimethylformamide.

Li S, Wang C
Journal of biochemical and molecular toxicology 2018 Sep;32(9):e22190

Ginsenoside Rg1 protects against acetaminophen-induced liver injury via activating Nrf2 signaling pathway in vivo and in vitro.

Ning C, Gao X, Wang C, Kong Y, Liu Z, Sun H, Sun P, Huo X, Ma X, Meng Q, Liu K
Regulatory toxicology and pharmacology : RTP 2018 Oct;98:58-68

Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS₂ nanoflowers.

Bai D, Li Q, Wang J, Zhao J, Deng X, Yuan L, Wu P
Toxicology research 2018 Nov 1;7(6):1267-1279

Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.

Li B, Li D, Wang Y, Meng X, Sun X, Tian J, Shi L, Ma F
Experimental animals 2018 Nov 1;67(4):451-461

Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.

Sun Y, Li TY, Song L, Zhang C, Li J, Lin ZZ, Lin SC, Lin SY
Hepatology (Baltimore, Md.) 2018 Jun;67(6):2397-2413

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Wang C, Xu W, Zhang Y, Huang D, Huang K
Cell death & disease 2018 Jul 26;9(8):819

Salvianolic acid A alleviates chronic ethanol-induced liver injury via promotion of β-catenin nuclear accumulation by restoring SIRT1 in rats.

Shi X, Zhao Y, Ding C, Wang Z, Ji A, Li Z, Feng D, Li Y, Gao D, Zhou J, Tian X, Yao J
Toxicology and applied pharmacology 2018 Jul 1;350:21-31

Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.

Zhang XB, Zeng YM, Chen XY, Zhang YX, Ding JZ, Xue C
Journal of thoracic disease 2018 Feb;10(2):825-834

Hepatoprotective Effect of Polyphenol-Enriched Fraction from Folium Microcos on Oxidative Stress and Apoptosis in Acetaminophen-Induced Liver Injury in Mice.

Wu H, Zhang G, Huang L, Pang H, Zhang N, Chen Y, Wang G
Oxidative medicine and cellular longevity 2017;2017:3631565

Glycyrrhetinic acid prevents acetaminophen-induced acute liver injury via the inhibition of CYP2E1 expression and HMGB1-TLR4 signal activation in mice.

Yang G, Zhang L, Ma L, Jiang R, Kuang G, Li K, Tie H, Wang B, Chen X, Xie T, Gong X, Wan J
International immunopharmacology 2017 Sep;50:186-193

Apigenin protects against alcohol-induced liver injury in mice by regulating hepatic CYP2E1-mediated oxidative stress and PPARα-mediated lipogenic gene expression.

Wang F, Liu JC, Zhou RJ, Zhao X, Liu M, Ye H, Xie ML
Chemico-biological interactions 2017 Sep 25;275:171-177

Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders.

Wang H, Xu YS, Wang ML, Cheng C, Bian R, Yuan H, Wang Y, Guo T, Zhu LL, Zhou H
International journal of molecular medicine 2017 Apr;39(4):819-830

Chronic Acrylamide Exposure in Male Mice Results in Elevated DNA Damage in the Germline and Heritable Induction of CYP2E1 in the Testes.

Katen AL, Chambers CG, Nixon B, Roman SD
Biology of reproduction 2016 Oct 1;95(4):86

Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation.

Ren Z, Yang F, Wang X, Wang Y, Xu M, Frank JA, Ke ZJ, Zhang Z, Shi X, Luo J
Toxicology and applied pharmacology 2016 Oct 1;308:11-19

Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress.

Shi H, Shi A, Dong L, Lu X, Wang Y, Zhao J, Dai F, Guo X
Clinical nutrition (Edinburgh, Scotland) 2016 Dec;35(6):1366-1373

Hepatoprotective and antioxidant effects of lycopene on non-alcoholic fatty liver disease in rat.

Jiang W, Guo MH, Hai X
World journal of gastroenterology 2016 Dec 14;22(46):10180-10188

Alteration in the expression of cytochrome P450s (CYP1A1, CYP2E1, and CYP3A11) in the liver of mouse induced by microcystin-LR.

Zhang B, Liu Y, Li X
Toxins 2015 Mar 30;7(4):1102-15

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Dong D, Yin L, Qi Y, Xu L, Peng J
Nutrients 2015 Jun 15;7(6):4829-50

Potent effects of dioscin against obesity in mice.

Liu M, Xu L, Yin L, Qi Y, Xu Y, Han X, Zhao Y, Sun H, Yao J, Lin Y, Liu K, Peng J
Scientific reports 2015 Jan 22;5:7973

Potent effects of dioscin against liver fibrosis.

Zhang X, Han X, Yin L, Xu L, Qi Y, Xu Y, Sun H, Lin Y, Liu K, Peng J
Scientific reports 2015 Apr 8;5:9713

Dioscin attenuates hepatic ischemia-reperfusion injury in rats through inhibition of oxidative-nitrative stress, inflammation and apoptosis.

Tao X, Wan X, Xu Y, Xu L, Qi Y, Yin L, Han X, Lin Y, Peng J
Transplantation 2014 Sep 27;98(6):604-11

Ursolic acid protects mouse liver against CCl4-induced oxidative stress and inflammation by the MAPK/NF-κB pathway.

Ma JQ, Ding J, Zhang L, Liu CM
Environmental toxicology and pharmacology 2014 May;37(3):975-83

Protective effects of dioscin against alcohol-induced liver injury.

Xu T, Zheng L, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J
Archives of toxicology 2014 Mar;88(3):739-53

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Dong D, Qi Y, Xu L, Yin L, Xu Y, Han X, Zhao Y, Peng J
Food & function 2014 Dec;5(12):3065-3075

Potent effects of flavonoid-rich extract from Rosa laevigata Michx fruit against hydrogen peroxide-induced damage in PC12 cells via attenuation of oxidative stress, inflammation and apoptosis.

Liu M, Xu Y, Han X, Liang C, Yin L, Xu L, Qi Y, Zhao Y, Peng J, Sun C
Molecules (Basel, Switzerland) 2014 Aug 7;19(8):11816-32

Human placental lactogen induces CYP2E1 expression via PI 3-kinase pathway in female human hepatocytes.

Lee JK, Chung HJ, Fischer L, Fischer J, Gonzalez FJ, Jeong H
Drug metabolism and disposition: the biological fate of chemicals 2014 Apr;42(4):492-9

Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation.

Yu H, Zheng L, Yin L, Xu L, Qi Y, Han X, Xu Y, Liu K, Peng J
International immunopharmacology 2014 Apr;19(2):233-44

Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice.

Dong D, Zhang S, Yin L, Tang X, Xu Y, Han X, Qi Y, Peng J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Dec;62:120-30

Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.

Zhang S, Zheng L, Dong D, Xu L, Yin L, Qi Y, Han X, Lin Y, Liu K, Peng J
Food chemistry 2013 Dec 1;141(3):2108-16

Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells.

Song J, Zhao M, Liu X, Zhu Y, Hu X, Chen F
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2013 Aug;58:306-10

Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.

Zhao X, Cong X, Zheng L, Xu L, Yin L, Peng J
Toxicology letters 2012 Oct 2;214(1):69-80

Protective effect of L-theanine on carbon tetrachloride-induced acute liver injury in mice.

Jiang W, Gao M, Sun S, Bi A, Xin Y, Han X, Wang L, Yin Z, Luo L
Biochemical and biophysical research communications 2012 Jun 1;422(2):344-50

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse liver tissue were subjected to SDS PAGE followed by western blot with 19937-1-AP(CYP2E1-Specific antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CYP2E1 antibody 19937-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line