14342-1-AP

antibody from Invitrogen Antibodies

Targeting: DIAPH3 AN, AUNA1, DRF3, FLJ34705, NSDAN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14342-1-AP

Provider

Invitrogen Antibodies

Product name

DIAPH3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DQEQLNSLS QFKSEYSNLC EPEQFVVVMS NVKRLRPRLS AILFKLQFEE QVNNIKPDIM AVSTACEEIK KSKSFSKLLE LVLLMGNYMN AGSRNAQTFG FNLSSLCKLK DTKSADQKTT LLHFLVEICE EKYPDILNFV DDLEPLDKAS KVSVETLEKN LRQMGRQLQQ LEKELETFPP PEDLHDKFVT KMSRFVISAK EQYETLSKLH ENMEKLYQSI IGYYAIDVKK VSVEDFLTDL NNFRTTFMQA IKENIKKREA EEKEKRVRIA KELAERERLE RQQKKKRLLE MKTEGDETGV MDNLLEALQS GAAFRDRRKR TPMPKDVRQS LSPMSQRPVL KVCNHGNKPY L (500-850 aa encoded by BC048963)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP ( DIAPH3 Antibody) at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug.