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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA500702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SORD Monoclonal Antibody (OTI1D5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1D5
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100 µg) and LC401082 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-SORD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SORD mouse monoclonal antibody (TA500702) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SORD(RC200415).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SORD mouse monoclonal antibody (TA500702).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY SORD(RC200415)(Red) or empty vector control plasmid (Blue) were immunostained with anti-SORD mouse monoclonal(TA500702, Dilution 1:1,000), and then analyzed by flow cytometry.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-SORD antibody (TA500702), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).
