NB100-2466

antibody from Novus Biologicals

Targeting: ITPR2 CFAP48, IP3R2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2466

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2466, RRID:AB_2280741

Product name

Goat Polyclonal ITPR2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. No cross-reactivity expected with other types of ITPR.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Nuclear calcineurin is a sensor for detecting Ca²⁺ release from the nuclear envelope via IP₃R.

Olivares-Florez S, Czolbe M, Riediger F, Seidlmayer L, Williams T, Nordbeck P, Strasen J, Glocker C, Jänsch M, Eder-Negrin P, Arias-Loza P, Mühlfelder M, Plačkić J, Heinze KG, Molkentin JD, Engelhardt S, Kockskämper J, Ritter O
Journal of molecular medicine (Berlin, Germany) 2018 Nov;96(11):1239-1249

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Role of subcellular calcium redistribution in regulating apoptosis and autophagy in cadmium-exposed primary rat proximal tubular cells.

Liu F, Li ZF, Wang ZY, Wang L
Journal of inorganic biochemistry 2016 Nov;164:99-109

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Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead.

Wang H, Wang ZK, Jiao P, Zhou XP, Yang DB, Wang ZY, Wang L
Toxicology 2015 Jul 3;333:137-146

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Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure.

Ljubojevic S, Radulovic S, Leitinger G, Sedej S, Sacherer M, Holzer M, Winkler C, Pritz E, Mittler T, Schmidt A, Sereinigg M, Wakula P, Zissimopoulos S, Bisping E, Post H, Marsche G, Bossuyt J, Bers DM, Kockskämper J, Pieske B
Circulation 2014 Jul 15;130(3):244-55

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Glucocorticoids downregulate Fyn and inhibit IP(3)-mediated calcium signaling to promote autophagy in T lymphocytes.

Harr MW, McColl KS, Zhong F, Molitoris JK, Distelhorst CW
Autophagy 2010 Oct;6(7):912-21

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IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism.

Futatsugi A, Nakamura T, Yamada MK, Ebisui E, Nakamura K, Uchida K, Kitaguchi T, Takahashi-Iwanaga H, Noda T, Aruga J, Mikoshiba K
Science (New York, N.Y.) 2005 Sep 30;309(5744):2232-4

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours at room temperature at 1:5000. (Data provided by Karen McColl at Case Western Reserve University)

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.