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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-97677 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DUOX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.837 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DUOX2 using a DUOX2 Polyclonal antibody (Product # PA5-97677) at a concentration of 2.18 µg/mL. Lane 1: Jurkat whole cell lysate. Lane 2: HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 176 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DUOX2 using a DUOX2 Polyclonal antibody (Product # PA5-97677). Positive WB detected in: 293 whole cell lysate. All lanes: DUOX2 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 176 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DUOX2 in multiple sample types using a DUOX2 polyclonal antibody (Product # PA5-97677) at a dilution of 1:500. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, and Ntera-2 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DUOX2 in Jurkat cells. The sample was incubated with a DUOX2 polyclonal antibody (Product # PA5-97677), followed by FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DUOX2 in Jurkat cells. The sample was incubated with a DUOX2 polyclonal antibody (Product # PA5-97677), followed by FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DUOX2 in paraffin embedded human small intestine tissue using a DUOX2 polyclonal antibody (Product # PA5-97677) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DUOX2 in paraffin-embedded human small intestine tissue. The sample was incubated with a DUOX2 polyclonal antibody (Product # PA5-97677) at a dilution of 1:50.
