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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27226 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TID1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116.
- Concentration
- 0.46 mg/mL
Submitted references Essential role of TID1 in maintaining mitochondrial membrane potential homogeneity and mitochondrial DNA integrity.
Ng AC, Baird SD, Screaton RA
Molecular and cellular biology 2014 Apr;34(8):1427-37
Molecular and cellular biology 2014 Apr;34(8):1427-37
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TID1 Polyclonal Antibody (Product # PA5-27226). Sample (30 µg of whole cell lysate). Lane A: HepG2. Lane B: HCT116. 10% SDS PAGE. TID1 Polyclonal Antibody (Product # PA5-27226) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TID1 in methanol-fixed HeLa cells using a TID1 polyclonal antibody (Product # PA5-27226) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using TID1 (Product # PA5-27226) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
