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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- CF500572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POR Monoclonal Antibody (OTI3H10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3H10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POR (RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-POR. Positive lysates LY424436 (100 µg) and LC424436 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POR (RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-POR. Positive lysates LY424436 (100 µg) and LC424436 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-POR mouse monoclonal antibody (TA500572) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POR(RC202172).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-POR mouse monoclonal antibody (TA500572) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POR(RC202172).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500572, Dilution 1:50)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500572, Dilution 1:50)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY POR(RC202172)(Red) or empty vector control plasmid (Blue) were immunostained with anti-POR mouse monoclonal(TA500572, Dilution 1:1,000), and then analyzed by flow cytometry.
