F42027

antibody from NSJ Bioreagents

Targeting: SUMO3 SMT3A, SMT3H1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F42027

Provider

NSJ Bioreagents

Product name

SUMO2/3 Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 49-81 from human SUMO3 was used as the immunogen for this SUMO2/3 antibody.

Description

Purified antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the SUMO2/3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Top: lysate tested with ERM Ab. Center: IP with FLAG Ab followed by WB with NSJ# F42008 SUMO antibody. Bottom: IP with FLAG Ab followed by WB with NSJ# F42027 SUMO2/3 antibody. (*) represents immunoprecipitated ERM-like forms detected by SUMO Abs.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) 293T, (2) HeLa, (3) HL-60, (4) Jurkat cell lines and (5) rat liver tissue using SUMO2/3 antibody at 1:1000.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SUMO2/3 antibody western blot analysis in (10 U251 cells, (2) mouse liver and (3) rat liver lysate.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of SUMO2/3 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO3 gene.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of SH-SY5Y cells stained with SUMO2/3 antibody diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of HeLa cells stained with SUMO2/3 antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of U251 cells stained with SUMO2/3 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human hepatocarcinoma stained with SUMO2/3 antibody.