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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 15007-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LLPAIYLME NVAMEELITK QRKSKEIVEE AIRCKLKILQ NDGVVTSLCA RPRKTGHALF LLGGQTFMCD KLYLVDQKAK EIIPKADIPS PRKEFSACAI GCKVYITGGR GSENGVSKDV WVYDTLHEEW SKAAPMLVAR FGHGSAELKH CLYVVGGHTA ATGCLPASPS VSLKQVEHYD PTINKWTMVA PLREGVSNAA VVSAKLKLFA FGGTSVSHDK LPKVQCYDQC ENRWTVPATC PQPWRYTAAA VLGNQIFIMG GDTEFSACSA YKFNSETYQW TKVGDVTAKR MSCHAVASGN KLYVVGGYFG IQRCKTLDCY DPTLDVWNSI TTVPYSLIPT AFVSTWKHLP S (240-589 aa encoded by BC000418)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ENC1 (IP:15007-1-AP, 4ug; Detection:15007-1-AP 1:1000) with mouse brain tissue lysate 2640ug.
