PA5-36146

antibody from Invitrogen Antibodies

Targeting: HEXB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36146

Provider

Invitrogen Antibodies

Product name

HEXB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 63 kDa.

Concentration

1 mg/mL

References

Submitted references

Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model.

Song Z, Xu Y, Zhang L, Zhou L, Zhang Y, Han Y, Li X, Yu P, Qu Y, Zhao W, Qin C
Frontiers in genetics 2020;11:565479

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Hexb using Hexb polyclonal antibody (Product # PA5-36146) at a dilution of 1:500. Lane 1: Jurkat whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HEXB in Lane 1: A549 whole cell lysate (40 µg), Lane 2: MCF-7 whole cell lysate (40 µg), Lane 3: U-87MG whole cell lysate (40 µg). Samples were incubated with HEXB polyclonal antibody (Product # PA5-36146) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HEXB in paraffin-embedded human breast carcinoma tissue. Samples were incubated with HEXB polyclonal antibody (Product # PA5-36146) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 Western blots (WB) and quantitative analysis of 11 differential proteins in the urinary exosomes isolated from control and 5XFAD mice. (A) Same aliquot of urinary exosomes isolated from three control samples and three 5XFAD mice samples. AOAH, Clusterin, Ceruloplasmin, Cathepsin B, ENPP2, FUCA2, HEXB, Ly86, Pcolce, Preol, and PrP proteins were detected by WB in sequence. Representative blots of the tested proteins and the molecular weight of standard proteins were presented. (B-L) Quantitative analysis of each of the tested protein's relative fold change, respectively. All data are presented as mean +- SD in triplicate experiments; Student's t -test; * P < 0.05; ** P < 0.01.