Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [4]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 12575-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTTG1IP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (Product # 12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug.