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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM09375PU-N - Provider product page
- Provider
- OriGene
- Product name
- TUBB2B mouse monoclonal antibody, clone AT5B3, Purified
- Antibody type
- Monoclonal
- Description
- TUBB2B mouse monoclonal antibody, clone AT5B3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TUBB2B
- Isotype
- IgG
- Antibody clone number
- AT5B3
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis: Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human TUBB2b antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- IHC
