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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB9717 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB9717, RRID:AB_10774740
- Product name
- TUBB2B monoclonal antibody, clone AT5B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant TUBB2B.
- Isotype
- IgG
- Antibody clone number
- AT5B3
- Storage
- Store at 4°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates (35 ug) with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa.Lane 2 : 293T.Lasne 3 : mouse brain.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human SW480 cells stained with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) at 1 : 250 dilution with Texas Red (Red).Arrow head : Mitotic cell.Arrow : MTOC (microtubule organizing center).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with TUBB2B monoclonal antibody, clone AT5B3 (Cat # MAB9717) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
