TA324899

antibody from OriGene

Targeting: MOB1B MOB4A, MOBKL1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324899

Provider

OriGene

Product name

Rabbit polyclonal MOB4A Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal MOB4A Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

MOB1B

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term) (Cat. #TA324899). TA324899 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of NIH/3T3 cell stained with MOB4A Antibody (C-term)(Cat#TA324899).NIH/3T3 cells were incubated with MOB4A primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).MOB4A immunoreactivity is localized to Cytoplasm significantly.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) (Cat.#TA324899), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

IHC