PA5-55072

antibody from Invitrogen Antibodies

Targeting: LURAP1L bA3L8.2, C9orf150, FLJ38505, LRAP35b, MGC46502

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55072

Provider

Invitrogen Antibodies

Product name

LURAP1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KVPESLVRSL RGEEPVPRER DRDPCGGSGG GGGGGGGCSS SSSYCSFPPS LSSSSSSSPT SGSPRGSHSS ALERLETKLH LLRQEMVNLR ATDVRLMRQL LVINESIESI KW

Concentration

0.1 mg/mL

References

Submitted references

PDGF-BB regulates the transformation of fibroblasts into cancer-associated fibroblasts via the lncRNA LURAP1L-AS1/LURAP1L/IKK/IκB/NF-κB signaling pathway.

Ren X, Li L, Wu J, Lin K, He Y, Bian L
Oncology letters 2021 Jul;22(1):537

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LURAP1L in control (vector only transfected HEK293T lysate) and LURAP1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a LURAP1L Polyclonal Antibody (Product # PA5-55072).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of LURAP1L in human cell line A-431 shows positivity in nucleoli. Samples were probed using a LURAP1L Polyclonal Antibody (Product # PA5-55072).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LURAP1L in human testis tissue shows strong cytoplasmic positivity in cells of seminiferus ducts. Samples were probed using a LURAP1L Polyclonal Antibody (Product # PA5-55072).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Result of the target gene prediction and dual-luciferase reporter assay. The cells were treated with 30 ng/ml PDGF-BB in 10% fetal bovine serum for 72 h for three passages. (A) Reverse transcription-quantitative PCR analysis was performed to evaluate the expression of LURAP1L. Data are presented as the mean +- SEM of triplicate experiments. (B) Western blot analysis of LURAP1L. Densitometry was used to determine the LURAP1L/beta-actin ratios. Data are presented as means +- SEM of three independent experiments. (C) Dual-luciferase reporter assay was performed to verify the regulatory effect of LURAP1L-AS1 on LURAP1L. *P