NBP1-06640

antibody from Novus Biologicals

Targeting: KDM6B JMJD3, KIAA0346

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP1-06640

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-06640, RRID:AB_1582401

Product name

Rabbit Polyclonal Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Jmjd3 regulates inflammasome activation and aggravates DSS-induced colitis in mice.

Huang M, Wang Q, Long F, Di Y, Wang J, Zhun Zhu Y, Liu X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4107-4119

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Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.

Lu H, Xie Y, Tran L, Lan J, Yang Y, Murugan NL, Wang R, Wang YJ, Semenza GL
The Journal of clinical investigation 2020 Jul 27;

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Endogenous hydrogen sulfide regulates histone demethylase JMJD3-mediated inflammatory response in LPS-stimulated macrophages and in a mouse model of LPS-induced septic shock.

Liu S, Wang X, Pan L, Wu W, Yang D, Qin M, Jia W, Xiao C, Long F, Ge J, Liu X, Zhu Y
Biochemical pharmacology 2018 Mar;149:153-162

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Critical role of histone demethylase Jumonji domain-containing protein 3 in the regulation of neointima formation following vascular injury.

Luo X, Yang D, Wu W, Long F, Xiao C, Qin M, Law BY, Suguro R, Xu X, Qu L, Liu X, Zhu YZ
Cardiovascular research 2018 Dec 1;114(14):1894-1906

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Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Luo T, Dunphy PS, McBride JW
Frontiers in cellular and infection microbiology 2017;7:178

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The H3K27me3-demethylase KDM6A is suppressed in breast cancer stem-like cells, and enables the resolution of bivalency during the mesenchymal-epithelial transition.

Taube JH, Sphyris N, Johnson KS, Reisenauer KN, Nesbit TA, Joseph R, Vijay GV, Sarkar TR, Bhangre NA, Song JJ, Chang JT, Lee MG, Soundararajan R, Mani SA
Oncotarget 2017 Sep 12;8(39):65548-65565

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Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation.

Pham D, Yu Q, Walline CC, Muthukrishnan R, Blum JS, Kaplan MH
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):902-11

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-JMJD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Analysis of JMJD3 expression on HepG2 whole cell lysates.