20760002-0.1mg

antibody from Novus Biologicals

Targeting: STOML3 Epb7.2l, SRO

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20760002-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal STOML3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This product is specific for Human STOML3.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STOML3 Antibody [20760002] - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STOML3 Antibody [20760002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: STOML3 Antibody [20760002]

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: STOML3 Antibody [20760002] - Cells in lymphoid follicles were negative. Endothelial cells were distinctly stained. Strong membranous positivity was observed in fallopian tube, hepatocytes and gallbladder. Among malignancies, most tissues displayed moderate cytoplasmic staining. Distinct additional membranous positivity was observed in several adenocarcinomas of colon, breast, cervix, liver, ovary and also in melanomas. Image and statement courtesy of the Human Protein Atlas (HPA).