Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31504 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEPT12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PRSS50 is a testis protease responsible for proper sperm tail formation and function.
Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ
Development (Cambridge, England) 2021 Apr 15;148(8)
Development (Cambridge, England) 2021 Apr 15;148(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEPT12 Polyclonal Antibody (Product # PA5-31504). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 10% SDS PAGE. SEPT12 Polyclonal Antibody (Product # PA5-31504) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SEPT12 showing staining in the cytoskeleton of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a SEPT12 polyclonal antibody (Product # PA5-31504) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEPT12 Polyclonal Antibody detects SEPT12 protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: SEPT12 protein stained by SEPT12 Polyclonal Antibody (Product # PA5-31504) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded hela xenograft, using SEPT12 (Product # PA5-31504) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.