PA5-119935

antibody from Invitrogen Antibodies

Targeting: ARHGEF18 KIAA0521, MGC15913, P114-RhoGEF, p114RhoGEF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119935

Provider

Invitrogen Antibodies

Product name

ARHGEF18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate, rat kidney tissue, Hela.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate. Lane 2: Hela cell lysate. Lane 3: 293 cell lysate. Lane 4: PC-3 cell lysate. Lane 5: Daudi cell lysate. Predicted band size: 152 kDa. Observed band size: 114/152 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate. Lane 2: Hela cell lysate. Lane 3: 293 cell lysate. Lane 4: PC-3 cell lysate. Lane 5: Daudi cell lysate. Predicted band size: 152 kDa. Observed band size: 114/152 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat kidney tissue using ARHGEF18 Polyclonal Antibody (Product # PA5-119935). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ARHGEF18 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ARHGEF18 in HeLa cells using ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1 µg/mL (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at 4°C for 1 hour, the cells were stained with a Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at 4°C (dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).