Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 620002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#620002, RRID:AB_2716039
- Product name
- Purified anti-ATG17
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16188A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total lysates (15 µg protein) from HeLa (Lane 1), 10nM scrambled siRNA (lane 2), 1nM RB1CC1 siRNA (Lane 3) and 10nM RB1CC1 siRNA treated HeLa cells (Lane 4), 293T (Lane 5), 10nM scrambled siRNA (lane 6), 1nM RB1CC1 siRNA (Lane 7) and 10nM RB1CC1 siRNA treated 293T cells (Lane 8) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RB1CC1 antibody (clone W16188A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
- Conjugate
- Unconjugated