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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- GTX107702 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107702, RRID:AB_1240906
- Product name
- GOLPH2 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.
Liu KK, Qiu WR, Naveen Raj E, Liu HF, Huang HS, Lin YW, Chang CJ, Chen TH, Chen C, Chang HC, Hwang JK, Chao JI
Autophagy 2017 Jan 2;13(1):187-200
Autophagy 2017 Jan 2;13(1):187-200
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: HeLa membrane 10% SDS PAGE GTX107702 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOLPH2 antibody detects GOLPH2 protein by western blot analysis.A. 30 £gg HeLa whole cell lysate/extract B. 30 £gg HeLa membrane fraction lysate/extract10 % SDS-PAGEGOLPH2 antibody (GTX107702) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(GTX107702) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(GTX107702) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000.Red: Phalloidin, a F-actin marker.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.
