H00051280-M06
antibody from Abnova Corporation
Targeting: GOLM1
bA379P1.3, C9orf155, FLJ23608, GOLPH2, GP73
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00051280-M06 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051280-M06, RRID:AB_534883
- Product name
- GOLM1 monoclonal antibody (M06), clone 5B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant GOLM1.
- Antigen sequence
VQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAA
GEGRNQQKLRGEDDYNMDENEAESETDKQAALAGN
DRNIDVFNVEDQKRDTINLLDQREKRNHTL- Isotype
- IgG
- Antibody clone number
- 5B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches.
ERG rearrangement in small cell prostatic and lung cancer.
GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics.
GOLPH2 and MYO6: putative prostate cancer markers localized to the Golgi apparatus.
Braun M, Menon R, Nikolov P, Kirsten R, Petersen K, Schilling D, Schott C, Gündisch S, Fend F, Becker KF, Perner S
BMC cancer 2011 Dec 7;11:511
BMC cancer 2011 Dec 7;11:511
ERG rearrangement in small cell prostatic and lung cancer.
Scheble VJ, Braun M, Wilbertz T, Stiedl AC, Petersen K, Schilling D, Reischl M, Seitz G, Fend F, Kristiansen G, Perner S
Histopathology 2010 Jun;56(7):937-43
Histopathology 2010 Jun;56(7):937-43
GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics.
Kristiansen G, Fritzsche FR, Wassermann K, Jäger C, Tölls A, Lein M, Stephan C, Jung K, Pilarsky C, Dietel M, Moch H
British journal of cancer 2008 Sep 16;99(6):939-48
British journal of cancer 2008 Sep 16;99(6):939-48
GOLPH2 and MYO6: putative prostate cancer markers localized to the Golgi apparatus.
Wei S, Dunn TA, Isaacs WB, De Marzo AM, Luo J
The Prostate 2008 Sep 15;68(13):1387-95
The Prostate 2008 Sep 15;68(13):1387-95
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10.Lane 1: GOLM1 transfected lysate(45.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GOLM1 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GOLM1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to GOLM1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol