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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347097 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal CDYL Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal CDYL Antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 100ul
- Concentration
- Not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against CDYL. diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 66.5 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CDYL. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,700.
- Validation comment
- ELISA
