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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA3-074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF3 Monoclonal Antibody (39TA-2F5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA3-074 has been succesfully used in an immunofluorescence application.
- Antibody clone number
- 39TA-2F5
- Concentration
- Conc. Not Determined
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of TAF3 in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a TAF3 mouse monoclonal antibody (Product # MA3-074) at a dilution of 1:250 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
