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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- GTX100479 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100479, RRID:AB_1240396
- Product name
- ABAT antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX100479 diluted at 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein by Western blot analysis.A. 50 µg Rat brain lysate/extract7.5 % SDS-PAGEABAT antibody (GTX100479) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein by western blot analysis.A. 30 ?g SK-N-SH whole cell lysate/extract7.5% SDS-PAGEABAT antibody (GTX100479) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody (GTX100479) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ABAT antibody (GTX100479) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ABAT antibody (GTX100479) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ABAT antibody (GTX100479) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABAT antibody detects ABAT protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
