LS-C676281

antibody from LSBio

Targeting: PMM2 CDG1, CDG1a, CDGS, PMI, PMI1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C676281

Provider

LSBio

Product name

PMM2 Antibody LS-C676281

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes: PMM2 antibody at 3.8ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG-2 whole cell lysate Lane 3: MCF7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29,14 kDa Observed band size: 28 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating PMM2 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating PMM2 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)