NBP1-88662

antibody from Novus Biologicals

Targeting: B4GALNT1 beta1-4GalNAc-T, GALGT, SPG26

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-88662

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-88662, RRID:AB_11006565

Product name

Rabbit Polyclonal B4GALNT1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human B4GALNT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: B4GALNT1 Antibody [NBP1-88662] - Analysis in human cell line A-549.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: B4GALNT1 Antibody [NBP1-88662] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebral cortex shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining in human cerebral cortex and pancreas tissues using anti-B4GALNT1 antibody. Corresponding B4GALNT1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human lymph node.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebellum, cerebral cortex, lymph node and pancreas using Anti-B4GALNT1 antibody NBP1-88662 (A) shows similar protein distribution across tissues to independent antibody NBP1-88663 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human lymph node shows no positivity in germinal center cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.