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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005196-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005196-M01, RRID:AB_2162543
- Product name
- Mouse Monoclonal CXCL4/PF4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PF4 - platelet factor 4 (chemokine (C-X-C motif) ligand 4)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CXCL4/PF4 Antibody (3F6) [H00005196-M01] - Analysis of PF4 expression in transfected 293T cell line by PF4 monoclonal antibody (M01), clone 3F6.Lane 1: PF4 transfected lysate(10.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CXCL4/PF4 Antibody (3F6) [H00005196-M01] - Detection limit for recombinant GST tagged PF4 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CXCL4/PF4 Antibody (3F6) [H00005196-M01] - Analysis of PF4 transfected lysate using anti-PF4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PF4 MaxPab rabbit polyclonal antibody.
