13714-1-AP

antibody from Invitrogen Antibodies

Targeting: DMC1 LIM15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13714-1-AP

Provider

Invitrogen Antibodies

Product name

DMC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MKEDQVVAE EPGFQDEEES LFQDIDLLQK HGINVADIKK LKSVGICTIK GIQMTTRRAL CNVKGLSEAK VDKIKEAANK LIEPGFLTAF EYSEKRKMVF HITTGSQEFD KLLGGGIESM AITEAFGEFR TGKTQLSHTL CVTAQLPGAG GYPGGKIIFI DTENTL (1-165 aa encoded by B C035658)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 13714-1-AP (DMC1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 13714-1-AP (DMC1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 13714-1-AP (DMC1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DMC1 (IP:13714-1-AP, 3ug; Detection:13714-1-AP 1:600) with mouse testis tissue lysate 4800ug.