NBP1-84696

antibody from Novus Biologicals

Targeting: DYSF FER1L1, LGMD2B

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-84696 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-84696, RRID:AB_11039027
Product name: Rabbit Polyclonal Dysferlin Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human Dysferlin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

DYSF protein structure - NBP1-84696 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skeletal muscle shows high expression.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skin shows low expression as expected.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human testis.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Analysis in human skeletal muscle and skin tissues. Corresponding Dysferlin RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney, skeletal muscle, skin and testis using Anti-Dysferlin antibody NBP1-84696 (A) shows similar protein distribution across tissues to independent antibody NBP1-84695 (B).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney shows moderate to strong membranous positivity in cells in glomeruli and cells in tubules.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skeletal muscle shows strong cytoplasmic and membranous positivity in myocytes.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.