Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 15194-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15194-1-AP, RRID:AB_2091587
- Product name
- DHFR antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated DHFR antibody (Cat. #15194-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CYP3A5 unexpectedly regulates glucose metabolism through the AKT-TXNIP-GLUT1 axis in pancreatic cancer.
A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics.
Disease-linked mutations cause exposure of a protein quality control degron.
Proteasome granule formation is regulated through mitochondrial respiration and kinase signaling.
ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.
Establishment of a Dihydrofolate Reductase Gene Knock-In Zebrafish Strain to Aid Preliminary Analysis of Congenital Heart Disease Mechanisms.
Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.
mTORC1 stimulates cell growth through SAM synthesis and m(6)A mRNA-dependent control of protein synthesis.
Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.
Thymidylate synthase inhibitor raltitrexed can induce high levels of DNA damage in MYCN-amplified neuroblastoma cells.
A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.
Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.
Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins.
CXCR4 regulates Plasmodium development in mouse and human hepatocytes.
The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice.
Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21(waf1/cip1) expression.
'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.
Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells.
Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.
Shao M, Pan Q, Tan H, Wu J, Lee HW, Huber AD, Wright WC, Cho JH, Yu J, Peng J, Chen T
Genes & diseases 2024 Jul;11(4):101079
Genes & diseases 2024 Jul;11(4):101079
A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics.
Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST
Cellular and molecular gastroenterology and hepatology 2022;13(5):1554-1589
Cellular and molecular gastroenterology and hepatology 2022;13(5):1554-1589
Disease-linked mutations cause exposure of a protein quality control degron.
Kampmeyer C, Larsen-Ledet S, Wagnkilde MR, Michelsen M, Iversen HKM, Nielsen SV, Lindemose S, Caregnato A, Ravid T, Stein A, Teilum K, Lindorff-Larsen K, Hartmann-Petersen R
Structure (London, England : 1993) 2022 Sep 1;30(9):1245-1253.e5
Structure (London, England : 1993) 2022 Sep 1;30(9):1245-1253.e5
Proteasome granule formation is regulated through mitochondrial respiration and kinase signaling.
Waite KA, Roelofs J
Journal of cell science 2022 Sep 1;135(17)
Journal of cell science 2022 Sep 1;135(17)
ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.
Zhang T, Sun L, Hao Y, Suo C, Shen S, Wei H, Ma W, Zhang P, Wang T, Gu X, Li ST, Chen Z, Yan R, Zhang Y, Cai Y, Zhou R, Jia W, Huang F, Gao P, Zhang H
Nature cancer 2022 Jan;3(1):75-89
Nature cancer 2022 Jan;3(1):75-89
Establishment of a Dihydrofolate Reductase Gene Knock-In Zebrafish Strain to Aid Preliminary Analysis of Congenital Heart Disease Mechanisms.
Gong K, Xie T, Yang Y, Luo Y, Deng Y, Chen K, Tan Z, Guo H, Xie L
Frontiers in cardiovascular medicine 2021;8:763851
Frontiers in cardiovascular medicine 2021;8:763851
Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.
Wang Y, Lu H, Sun L, Chen X, Wei H, Suo C, Feng J, Yuan M, Shen S, Jia W, Wang Y, Zhang H, Li Z, Zhong X, Gao P
Cell death & disease 2021 Oct 2;12(10):902
Cell death & disease 2021 Oct 2;12(10):902
mTORC1 stimulates cell growth through SAM synthesis and m(6)A mRNA-dependent control of protein synthesis.
Villa E, Sahu U, O'Hara BP, Ali ES, Helmin KA, Asara JM, Gao P, Singer BD, Ben-Sahra I
Molecular cell 2021 May 20;81(10):2076-2093.e9
Molecular cell 2021 May 20;81(10):2076-2093.e9
Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.
Kollareddy M, Martinez LA
Cells 2021 Apr 2;10(4)
Cells 2021 Apr 2;10(4)
Thymidylate synthase inhibitor raltitrexed can induce high levels of DNA damage in MYCN-amplified neuroblastoma cells.
Yamashita K, Kiyonari S, Tsubota S, Kishida S, Sakai R, Kadomatsu K
Cancer science 2020 Jul;111(7):2431-2439
Cancer science 2020 Jul;111(7):2431-2439
A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.
Zhang X, Ruan C, Zhu H, Li K, Zhang W, Wang K, Hu L, Ye M
Proteomics 2020 Aug;20(15-16):e1900372
Proteomics 2020 Aug;20(15-16):e1900372
Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.
Chen J, Zhang H, Chen X
Antiviral research 2020 Aug;180:104825
Antiviral research 2020 Aug;180:104825
Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins.
Mensah EO, Guo XY, Gao XD, Fujita M
Journal of bioscience and bioengineering 2019 Oct;128(4):487-494
Journal of bioscience and bioengineering 2019 Oct;128(4):487-494
CXCR4 regulates Plasmodium development in mouse and human hepatocytes.
Bando H, Pradipta A, Iwanaga S, Okamoto T, Okuzaki D, Tanaka S, Vega-RodrÃguez J, Lee Y, Ma JS, Sakaguchi N, Soga A, Fukumoto S, Sasai M, Matsuura Y, Yuda M, Jacobs-Lorena M, Yamamoto M
The Journal of experimental medicine 2019 Aug 5;216(8):1733-1748
The Journal of experimental medicine 2019 Aug 5;216(8):1733-1748
The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice.
Li K, Li J, Gu J, Guo X, Gao T, Li D
Food & function 2018 May 23;9(5):2634-2643
Food & function 2018 May 23;9(5):2634-2643
Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21(waf1/cip1) expression.
Fei Z, Gao Y, Qiu M, Qi X, Dai Y, Wang S, Quan Z, Liu Y, Ou J
Journal of clinical biochemistry and nutrition 2016 Mar;58(2):105-13
Journal of clinical biochemistry and nutrition 2016 Mar;58(2):105-13
'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.
Calise SJ, Purich DL, Nguyen T, Saleem DA, Krueger C, Yin JD, Chan EK
Journal of cell science 2016 Aug 1;129(15):3042-52
Journal of cell science 2016 Aug 1;129(15):3042-52
Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells.
Yan KH, Lee LM, Hsieh MC, Yan MD, Yao CJ, Chang PY, Chen TL, Chang HY, Cheng AL, Lai GM, Chuang SE
Oncology reports 2013 Sep;30(3):1497-505
Oncology reports 2013 Sep;30(3):1497-505
Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.
Banka S, Blom HJ, Walter J, Aziz M, Urquhart J, Clouthier CM, Rice GI, de Brouwer AP, Hilton E, Vassallo G, Will A, Smith DE, Smulders YM, Wevers RA, Steinfeld R, Heales S, Crow YJ, Pelletier JN, Jones S, Newman WG
American journal of human genetics 2011 Feb 11;88(2):216-25
American journal of human genetics 2011 Feb 11;88(2):216-25
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The DHFR antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human DHFR. This antibody recognizes human,mouse,rat antigen. The DHFR antibody has been validated for the following applications: ELISA, WB analysis.