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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX117705 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX117705, RRID:AB_10618840
- Product name
- DHFR antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references High pemetrexed sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in pemetrexed resistant lung cancer cells with suppression by vinca alkaloids.
Kuo WT, Tu DG, Chiu LY, Sheu GT, Wu MF
Oncology reports 2017 Nov;38(5):2787-2795
Oncology reports 2017 Nov;38(5):2787-2795
The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in pemetrexed resistant lung cancer cells with suppression by vinca alkaloids.
Chiu LY, Hsin IL, Yang TY, Sung WW, Chi JY, Chang JT, Ko JL, Sheu GT
Oncogene 2017 Jan 12;36(2):242-253
Oncogene 2017 Jan 12;36(2):242-253
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 (GTX27909) 12% SDS PAGE GTX117705 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DHFR antibody detects DHFR protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract12% SDS-PAGEDHFR antibody (GTX117705) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using DHFR(GTX117705) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 £gg of DHFR antibody (GTX117705).Western blot analysis was performed using DHFR antibody (GTX117705).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DHFR(GTX117705) antibody at 1:500 dilution.
