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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00001719-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001719-M01, RRID:AB_2091585
- Product name
- Mouse Monoclonal Dihydrofolate Reductase/DHFR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. DHFR (2B10)
- Reactivity
- Human, Mouse, Rat, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Inhibiting homologous recombination decreases extrachromosomal amplification but has no effect on intrachromosomal amplification in methotrexate-resistant colon cancer cells.
The SGLT2 inhibitor empagliflozin improves the primary diabetic complications in ZDF rats.
Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats.
Recoupling of eNOS with folic acid prevents abdominal aortic aneurysm formation in angiotensin II-infused apolipoprotein E null mice.
The sodium-glucose co-transporter 2 inhibitor empagliflozin improves diabetes-induced vascular dysfunction in the streptozotocin diabetes rat model by interfering with oxidative stress and glucotoxicity.
Inflammatory monocytes determine endothelial nitric-oxide synthase uncoupling and nitro-oxidative stress induced by angiotensin II.
Divergent evolution of protein conformational dynamics in dihydrofolate reductase.
Influence of reduced folate carrier and dihydrofolate reductase genes on methotrexate-induced cytotoxicity.
Telomere capping in non-dividing yeast cells requires Yku and Rap1.
Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.
Cai M, Zhang H, Hou L, Gao W, Song Y, Cui X, Li C, Guan R, Ma J, Wang X, Han Y, Lv Y, Chen F, Wang P, Meng X, Fu S
International journal of cancer 2019 Mar 1;144(5):1037-1048
International journal of cancer 2019 Mar 1;144(5):1037-1048
The SGLT2 inhibitor empagliflozin improves the primary diabetic complications in ZDF rats.
Steven S, Oelze M, Hanf A, Kröller-Schön S, Kashani F, Roohani S, Welschof P, Kopp M, Gödtel-Armbrust U, Xia N, Li H, Schulz E, Lackner KJ, Wojnowski L, Bottari SP, Wenzel P, Mayoux E, Münzel T, Daiber A
Redox biology 2017 Oct;13:370-385
Redox biology 2017 Oct;13:370-385
Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats.
Jabs A, Oelze M, Mikhed Y, Stamm P, Kröller-Schön S, Welschof P, Jansen T, Hausding M, Kopp M, Steven S, Schulz E, Stasch JP, Münzel T, Daiber A
Vascular pharmacology 2015 Aug;71:181-91
Vascular pharmacology 2015 Aug;71:181-91
Recoupling of eNOS with folic acid prevents abdominal aortic aneurysm formation in angiotensin II-infused apolipoprotein E null mice.
Siu KL, Miao XN, Cai H
PloS one 2014;9(2):e88899
PloS one 2014;9(2):e88899
The sodium-glucose co-transporter 2 inhibitor empagliflozin improves diabetes-induced vascular dysfunction in the streptozotocin diabetes rat model by interfering with oxidative stress and glucotoxicity.
Oelze M, Kröller-Schön S, Welschof P, Jansen T, Hausding M, Mikhed Y, Stamm P, Mader M, Zinßius E, Agdauletova S, Gottschlich A, Steven S, Schulz E, Bottari SP, Mayoux E, Münzel T, Daiber A
PloS one 2014;9(11):e112394
PloS one 2014;9(11):e112394
Inflammatory monocytes determine endothelial nitric-oxide synthase uncoupling and nitro-oxidative stress induced by angiotensin II.
Kossmann S, Hu H, Steven S, Schönfelder T, Fraccarollo D, Mikhed Y, Brähler M, Knorr M, Brandt M, Karbach SH, Becker C, Oelze M, Bauersachs J, Widder J, Münzel T, Daiber A, Wenzel P
The Journal of biological chemistry 2014 Oct 3;289(40):27540-50
The Journal of biological chemistry 2014 Oct 3;289(40):27540-50
Divergent evolution of protein conformational dynamics in dihydrofolate reductase.
Bhabha G, Ekiert DC, Jennewein M, Zmasek CM, Tuttle LM, Kroon G, Dyson HJ, Godzik A, Wilson IA, Wright PE
Nature structural & molecular biology 2013 Nov;20(11):1243-9
Nature structural & molecular biology 2013 Nov;20(11):1243-9
Influence of reduced folate carrier and dihydrofolate reductase genes on methotrexate-induced cytotoxicity.
Yoon SA, Choi JR, Kim JO, Shin JY, Zhang X, Kang JH
Cancer research and treatment : official journal of Korean Cancer Association 2010 Sep;42(3):163-71
Cancer research and treatment : official journal of Korean Cancer Association 2010 Sep;42(3):163-71
Telomere capping in non-dividing yeast cells requires Yku and Rap1.
Vodenicharov MD, Laterreur N, Wellinger RJ
The EMBO journal 2010 Sep 1;29(17):3007-19
The EMBO journal 2010 Sep 1;29(17):3007-19
Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.
Ionova IA, Vásquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM
American journal of physiology. Heart and circulatory physiology 2008 Nov;295(5):H2178-87
American journal of physiology. Heart and circulatory physiology 2008 Nov;295(5):H2178-87
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of DHFR expression in transfected 293T cell line by DHFR monoclonal antibody (M01), clone 2B10.Lane 1: DHFR transfected lysate(21.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10 Analysis of DHFR expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Detection limit for recombinant GST tagged DHFR is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.
