PAB20658

antibody from Abnova Corporation

Targeting: DNAJC1 DNAJL1, ERdj1, MTJ1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20658

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20658, RRID:AB_10963170

Product name

DNAJC1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DNAJC1.

Antigen sequence

EDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVTDV
TTKAKQLKDSVTCSPGMVRLSELKSTVQNSRPIKT
ATTLPDDMITQREDAEGVAAEEEQEGDSGEQETGA
TDARPRRRKPARLLEATAKPEPEEKSRAKRQKDFD
IAEQNESS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DNAJC1 polyclonal antibody (Cat # PAB20658) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with DNAJC1 polyclonal antibody (Cat # PAB20658) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis with DNAJC1 polyclonal antibody (Cat # PAB20658) shows cytoplasmic and nuclear membrane positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)