BS-18539R

antibody from Invitrogen Antibodies

Targeting: CLEC16A Gop-1, KIAA0350

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-18539R

Provider

Invitrogen Antibodies

Product name

CLEC16A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: K562 cell lysates; Lane 2: Siha cell lysates; Lane 3: U2OS cell lysates; Lane 4: U87 mg cell lysates probed with CLEC16A Polyclonal Antibody, Unconjugated (bs-18539R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human K562 cells probed with CLEC16A Polyclonal Antibody, unconjugated (bs-18539R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: K562 cell lysates; Lane 2: Siha cell lysates; Lane 3: U2OS cell lysates; Lane 4: U87 mg cell lysates probed with CLEC16A Polyclonal Antibody, Unconjugated (bs-18539R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CLEC16A Polyclonal Antibody, Unconjugated (bs-18539R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CLEC16A Polyclonal Antibody, Unconjugated (bs-18539R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.