Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-53134 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYOF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: TIDLVIGYDP PSAPHPNDLS GPSVPGMGGD GEEDEGDEDR LDNAVRGPGP KGPVGTVSEA QLARRLTKVK NSRRMLSNKP QDFQIRVRVI EGRQLSGNNI RPVV
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYOF in mouse cell line NIH-3T3 and rat cell line NBT-II using a MYOF Polyclonal Antibody (Product # PA5-53134).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYOF in human cell lines A-431 and HEK293 using a MYOF Polyclonal Antibody (Product # PA5-53134). Corresponding MYOF RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MYOF in human cell line U-251 MG shows positivity in plasma membrane & vesicles. Samples were probed using a MYOF Polyclonal Antibody (Product # PA5-53134).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYOF in human cervix, uterine using MYOF Polyclonal Antibody (Product # PA5-53134) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYOF in human testis using MYOF Polyclonal Antibody (Product # PA5-53134) shows low expression as expected.