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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- 25318-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Desmoplakin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa).
- Concentration
- 0.4 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of Desmoplakin antibody (25318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Desmoplakin transfected HepG2 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 25318-1-AP (Desmoplakin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 25318-1-AP (Desmoplakin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 25318-1-AP (Desmoplakin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25318-1-AP ( Desmoplakin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25318-1-AP ( Desmoplakin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using (Product # 25318-1-AP) (Desmoplakin antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
