PB9478

antibody from Boster Biological Technology

Targeting: ASPH BAH, CASQ2BP1, HAAH, JCTN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9478

Provider

Boster Biological Technology

Product name

Anti-Aspartate beta hydroxylase/ASPH Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for aspartate beta hydroxylase/ASPH detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. aspartate beta hydroxylase/ASPH information: Molecular Weight: 85863 MW; Subcellular Localization: Isoform 1: Endoplasmic reticulum membrane; Single-pass type II membrane protein ; Tissue Specificity: Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Aspartate β-hydroxylase Regulates Expression of Ly6 Genes.

Kanwal M, Smahelova J, Ciharova B, Johari SD, Nunvar J, Olsen M, Smahel M
Journal of Cancer 2024;15(5):1138-1152

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ASPH using anti-ASPH antibody (PB9478). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: HELA Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.

Additional image