PA5-78827

ASPH antibody from Invitrogen Antibodies

BAH, CASQ2BP1, HAAH, JCTN

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Flow cytometry

Supportive data in Antibodypedia

Provider product page for PA5-78827

Antibody Data

Product number

PA5-78827

Provider

Invitrogen Antibodies

Product name

ASPH Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen.
Babich M, Sharma A, Li T, Radosevich JA
Heliyon 2022 Mar;8(3):e08988

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASPH in Lane 1: rat brain tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: HeLa whole cell lysate, Lane 4: HEPG2 whole cell lysate, Lane 5: HEPA whole cell lysate using 40-50 µg per well. Sample was incubated with ASPH (Product # PA5-78827) at a dilution of 0.5 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ASPH on paraffin-embedded human mammary cancer tissue. Sample was incubated with ASPH polyclonal antibody (Product# PA5-78827).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of ASPH in U87 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ASPH Polyclonal Antibody (Product # PA5-78827) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of ASPH in HeLa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ASPH Polyclonal Antibody (Product # PA5-78827) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.