- HPA043052 - Atlas Antibodies TIMM44 antibody

Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
Molecular and cellular biology 2015 Apr;35(7):1139-56

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Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting TIMM44 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting TIMM44 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:394
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod no: s20498
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 2: s20496
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s20497
Antibody Lot Number: R39348
Appendix: 565eed3d3c098.pdf

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TIMM44 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TIMM44 antibody. Remaining relative intensity is presented

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Western blot analysis in human cell lines PC-3 and HeLa using Anti-TIMM44 antibody. Corresponding TIMM44 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human heart muscle shows moderate to strong granular cytoplasmic positivity in cardiomyocytes.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human gastrointestinal shows moderate to strong granular cytoplasmic positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
Sample type: HUMAN

HPA043052