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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- 13859-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIMM44 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: AKTAKQSAE SVSKGGEKLG RTAAFRALSQ GVESVKKEID DSVLGQTGPY RRPQRLRKRT EFAGDKFKEE KVFEPNEEAL GVVLHKDSKW YQQWKDFKEN NVVFNRFFEM KMKYDESDNA FIRASRALTD KVTDLLGGLF SKTEMSEVLT EILRVDPAFD KDRFLKQCEN DIIPNVLEAM ISGELDILKD WCYEATYSQL AHPIQQAKAL GLQFHSRILD IDNVDLAMGK MMEQGPVLII TFQAQLVMVV RNPKGEVVEG DPDKVLRMLY VWALCRDQDE LNPYAAWRLL DISASSTEQI L (153-452 aa encoded by BC033628)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13859-1-AP ( TIMM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 13859-1-AP (TIMM44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13859-1-AP (TIMM44 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A549 cells, using TIMM44 antibody 13859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13859-1-AP (TIMM44 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TIMM44 (IP:13859-1-AP, 4ug; Detection:13859-1-AP 1:1000) with HeLa cells lysate 1280 ug.
