Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00027163-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00027163-M01, RRID:AB_536941
- Product name
- Mouse Monoclonal ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ASAHL - N-acylsphingosine amidohydrolase (acid ceramidase)-like
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - NAAA monoclonal antibody (M01), clone 5E3 Analysis of ASAHL expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Detection limit for recombinant GST tagged NAAA is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Analysis of monoclonal antibody to NAAA on NIH/3T3 cell. Antibody concentration 10 ug/ml.