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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX105658 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105658, RRID:AB_1950168
- Product name
- EEF1E1 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE GTX105658 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:A431(GTX27909)12% SDS PAGEGTX105658 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1(GTX105658) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
