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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-28283 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF1E1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Raji, mouse brain.
- Concentration
- 0.2 mg/mL
Submitted references Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response.
Jin D, Wek SA, Kudlapur NT, Cantara WA, Bakhtina M, Wek RC, Musier-Forsyth K
The Journal of biological chemistry 2021 Oct;297(4):101203
The Journal of biological chemistry 2021 Oct;297(4):101203
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF1E1 Polyclonal Antibody (Product # PA5-28283). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. EEF1E1 Polyclonal Antibody (Product # PA5-28283) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF1E1 Polyclonal Antibody (Product # PA5-28283). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 12% SDS PAGE. EEF1E1 Polyclonal Antibody (Product # PA5-28283) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EEF1E1 in paraformaldehyde-fixed HeLa cells using an EEF1E1 polyclonal antibody (Product # PA5-28283) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EEF1E1 Polyclonal Antibody detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 Polyclonal Antibody (Product # PA5-28283) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EEF1E1 Polyclonal Antibody detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 Polyclonal Antibody (Product # PA5-28283) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 MSC association and protein integrity of EPRS-FLAG protein overexpressed in HEK293 T cells. A , doxycycline-inducible EPRS knockdown in HEK293T cells using shEPRS ( left ) or a nonspecific shRNA ( right ). B , immunoprecipitation experiments with FLAG antibody in HEK293T cells with endogenous EPRS knockdown and WT and mutant EPRS-FLAG overexpression. EPRS ( top ) and MSC scaffold proteins AIMP2 ( middle ) and AIMP3 ( bottom ) were visualized by immunoblotting using the indicated antibodies.
