500-P134-100UG

antibody from Invitrogen Antibodies

Targeting: CCL25 Ckb15, SCYA25, TECK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P134-100UG

Provider

Invitrogen Antibodies

Product name

TECK Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Hepatic endothelial CCL25 mediates the recruitment of CCR9+ gut-homing lymphocytes to the liver in primary sclerosing cholangitis.

Eksteen B, Grant AJ, Miles A, Curbishley SM, Lalor PF, Hübscher SG, Briskin M, Salmon M, Adams DH
The Journal of experimental medicine 2004 Dec 6;200(11):1511-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human TECK (CCL25) by Western Blot analysis TECK Polyclonal Antibody (Product # 500-P134-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human TECK (CCL25) by Western Blot analysis TECK Polyclonal Antibody (Product # 500-P134-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TECK Polyclonal Antibody (Product # 500-P134-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TECK Polyclonal Antibody, Biotin (Product # 500-P134BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15).