DPABH-25600

antibody from Creative Diagnostics

Targeting: GSPT1 eRF3a, ETF3A, GST1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

DPABH-25600

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DPABH-25600, RRID:AB_2506490

Product name

Rabbit Anti-Human GSPT1 Polyclonal antibody

Antibody type

Polyclonal

Description

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID:15917414,12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of GSPT1 as a BIR3-binding proteinÿUsing a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1 protein, lacking the first 69 residues. (PMID: 12865429)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 uL