Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP51962PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51962PU-N, RRID:AB_11149714
- Product name
- anti GSPT1 / ERF3A (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of Human GSPT1 / ERF3A
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of GSPT1 / ERF3A antibody (N-term) Cat.-No AP51962PU-N in U251, CEM cell line lysates (35ug/lane). This demonstrates the GSPT1 antibody detected the GSPT1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma reacted with GSPT1 / ERF3A antibody (N-term) Cat.-No AP51962PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of U251 cells using GSPT1 / ERF3A antibody (N-term) Cat.-No AP51962PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.