Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6419 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Netrin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Netrin-1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O95631
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus.
The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration.
Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC).
Prieto CP, Casas BS, Falcón P, Villanueva A, Lois P, Lattus J, Palma V
International journal of molecular sciences 2019 Mar 20;20(6)
International journal of molecular sciences 2019 Mar 20;20(6)
The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration.
Villanueva AA, Falcón P, Espinoza N, R LS, Milla LA, Hernandez-SanMiguel E, Torres VA, Sanchez-Gomez P, Palma V
Oncotarget 2017 Feb 7;8(6):9767-9782
Oncotarget 2017 Feb 7;8(6):9767-9782
Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells (WJ-MSC).
Prieto CP, Ortiz MC, Villanueva A, Villarroel C, Edwards SS, Elliott M, Lattus J, Aedo S, Meza D, Lois P, Palma V
Stem cell research & therapy 2017 Feb 28;8(1):43
Stem cell research & therapy 2017 Feb 28;8(1):43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Netrin-1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Netrin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6419) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Netrin-1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.